RBC Pipette

The RBC pipette is diluting pipette also present inside the Hemocytometer The blood along with the RBC diluting fluid is drawn in the pipette and are mixed well through the red bead present inside the bulb of the pipette

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is diluting pipette also present inside the Hemocytometer The blood along with the RBC diluting fluid is drawn in the pipette and are mixed well through the red bead present inside the bulb of the pipette

A Pipette is a tool used in to dispense a measurable quantity of blood.   Various pipettes are used in  hematology which includes RBC & WBC pipettes, Hemoglobin pipette etc.   Here we will understand  the differences between RBC &  WBC pipettes and their various uses.

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1. What is the  other name of  RBC/WBC pipette  used to make dilutions of whole blood?
Thoma pipette

2. Describe the R-B-C Pipette
RBC pipette has red bead in the bulb and it has marking of 0.5, 1 and 101.

3. What is the role of red bead in pipette?
Helps in identification of pipette
Helps in mixing of diluting fluid and blood
To ensure dryness of pipette: Free mobility indicates a dry pipette

4. What are the other uses of RBC pipette?
It is used wherever higher dilution is required Eg; High total leukocyte count and total sperm count

5. Name the diluting fluid used for RBC count
Hayem’s fluid, Dacie’s fluid

6. What is the dilution used in RBC pipette?
1:200

7. What is the volume of blood the pipette delivers
It delievers 20ul or 0.02 ml of blood.
8. What is the composition of Hayem’s fluid
Hayem’s fluid: a. Sodium chloride -0.5 gm
b. Sodium sulfate -2.5 gm
c. Mercuric chloride -0.25 gm
d. Distilled water – 100 ml.

9. What is the composition of Dacie’s fluid:
Dacie’s fluid:
a. Trisodium citrate – 3 gm.
b. Formalin – 1 ml.
b. Distill water – 99 ml.

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RBC pipette, also known as a red blood cell pipette or hematocrit pipette, is a laboratory instrument used to measure the volume of red blood cells (RBCs) in a blood sample. It is a specialized pipette designed with a calibrated narrow tube and a bulbous reservoir at one end.

Here's how the RBC pipette is typically used:

1. Blood Collection: A small amount of blood is collected from the patient using a syringe or capillary tube. The blood is usually taken from a finger prick or a vein in the arm, depending on the specific test being conducted.

2. Filling the Pipette: The narrow end of the RBC pipette is immersed in the collected blood sample. By applying gentle suction or using the bulbous reservoir, blood is drawn up into the pipette, filling it to a predetermined volume.

3. Sealing: Once the blood reaches the desired volume, the pipette is removed from the blood sample, and the narrow end is sealed with a cap or plug. This ensures that the blood sample remains intact within the pipette.

4. Centrifugation: The sealed RBC pipette is then placed in a centrifuge, a machine that spins the pipette at high speeds. The centrifugal force separates the different components of the blood based on their density. RBCs tend to settle at the bottom due to their higher density.

5. Reading the Hematocrit: After centrifugation, the RBCs form a distinct red layer at the bottom of the pipette. The volume of this layer is measured against calibrated markings on the pipette. The measurement indicates the percentage of the total blood volume occupied by the RBCs, known as the hematocrit value.

The hematocrit value obtained using an RBC pipette is an essential parameter in assessing an individual's blood composition. It helps diagnose conditions such as anemia, polycythemia, and dehydration, as well as monitor the response to certain treatments.

It's worth noting that there may be slight variations in the design and usage of RBC pipettes depending on the specific manufacturer or laboratory protocol. Always follow the instructions provided by the manufacturer or the guidelines of the laboratory you are working in.