Dengue IgG Elisa 96T pack
Dengue IgG Elisa 96T pack is intended to be used for the qualitative detection of IgG anti-dengue viruses (DEN1,2,3,4) in human serum or plasma. It is intended to be used by professionals as an aid in the diagnosis of active infection with dengue viruses, including secondary infection
Dengue IgG Elisa 96T pack
Dengue IgG Elisa
ELISA INTENDED USE
TRUSTwell Dengue IgG ELISA Kit is a solid-phase, IgG capture enzyme linked immunosorbent assay. The three main kit components are
1) A 96 breakable well plate with the solid microwells pre-coated with mouse monoclonal anti-human IgG antibody
2) Recombinant dengue antigen
3) HRP-anti-dengue conjugates
This test is intended to be used for the qualitative detection of IgG anti-dengue viruses (DEN1,2,3,4) in human serum or plasma. It is intended to be used by professionals as an aid in the diagnosis of active infection with dengue viruses, including secondary infection. Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of health care providers.
Recommended for use:
Any person presenting with clinical signs or symptoms consistent with acute dengue infection or dengue fever including: high fever, severe headaches, pain behind the eyes, severe joint and muscle pain, fatigue, nausea, vomiting, and/or skin rash.
During primary infection, anti-dengue IgG antibodies become detectable by the end of the first week of illness. They increase slowly to peak around 14-21 days, and then decrease to low levels, persisting for the duration of life. During secondary infection, a strong and rapid IgG antibody response is often observed. IgG levels are detectable as early as 3 days after the onset of symptoms, with the peak detection window being 1-2 weeks after symptom onset. In secondary infection, IgG antibodies rise to levels higher than during primary infection. Importantly, anti-dengue IgG antibodies increase earlier than or simultaneously with IgM antibodies, and at much higher levels during secondary infection.
SeraCare AccuSet Performance Panel (0845-0051): 100% agreement with a market leading Dengue IgG Capture ELISA
Cross Reactivity: No cross reaction with HCV, Syphilis, HBsAg, ANA, HIV, HAMA, H. pylori, RF, Malaria
Interference: No interference with Salicylic acid: 4.34 mmol/L; Glucose: 55 mmol/L; Sodium citrate: 1.3 %;Heparin: 3,000 U/L; Creatinine: 5 mmol/L; Bilirubin: 10 mg/dL; EDTA: 3.4 ?mol/L
Intra Assay Precision: CV < 7%
Inter Assay Precision: CV < 6%
Shelf life: 18 months
Open vial stability: 8 weeks, or until the expiration date
Anti-human IgG coated microwells
Lyophilized dengue antigen
HRP-anti-dengue conjugates (100X concentrate)
Dengue IgG positive control
Dengue IgG negative control
Wash buffer (30X concentrate)
TMB substrate A
TMB substrate B
ELISA working sheet
|Dimensions||18 × 10 × 9 cm|
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